For this, the suitability of functional genes specific for and common to all EFF were evaluated. The aim of this study was to develop and evaluate an approach based on quantitative PCR (qPCR). To this end, a high-sensitivity detection method is still needed.
It is therefore desirable to assess their prevalence in environmental and industrial fields. In addition to natural habitats, EFF are often the cause of contamination problems in anthropogenic environments, such as industrial production plants or hospitals. EFF are considered ubiquitous in natural environments, in particular, those subjected to stress conditions. Bacterial endospores are highly specialized cellular forms that allow endospore-forming Firmicutes (EFF) to tolerate harsh environmental conditions.